Bluestain in wood can cause serious economic losses to the forest industry and may be, or be perceived as, a phytosanitary risk. The fungi causing Bluestain are commonly referred to as Ophiostomatoid fungi and include species of Ophiostoma, Grosmannia, and Ceratocystiopsis, from the order Ophiostomatales, as well as some species in Ceratocystis in the order Microascales. Many of these fungi are morphologically similar and cause similar symptoms, which makes traditional identification difficult. Identification and/or detection of these fungi are commonly done using molecular methodology. An understanding of the causal organism is necessary in the management of bluestain on forest products; therefore a review of the molecular work done in terms of classification, identification and detection on these fungi was undertaken in order to produce a reference guide for future identification/detection of bluestain fungi using molecular technology.