Wood decay is one of the primary factors limiting wood as a building material, reducing the competitiveness of wood against other engineered products. Wood preservatives or other wood enhancing treatments can reduce the risk of decay; however, Canadian wood species such as spruce and pine are difficult to treat to meet Canadian and USA standards due to a thin sapwood band and refractory heartwood. This puts the Canadian wood preservation industry at a competitive disadvantage in both domestic and international markets. Biological incising with Dichomitus squalens was originally done in Austria in the 1990s to increase the permeability of European spruce prior to treatment with wood preservatives. In 2010, FPInnovations screened Canadian isolates of various white-rot fungi to identify an isolate that would be suitable for biological incising of Canadian spruce and pine. An isolate of Dichomitus squalens isolated from white spruce was found to greatly increase the permeability of the wood, particularly in spruce. Follow up studies in 2011 and 2012 confirmed that the results could be reproduced on commercial sized wood in non-sterile conditions, and showed that longer times would be required for bioincising in pine which is more resistant to D. squalens. Strength loss on small clear specimens with through treatment was slightly higher in some samples than with conventional incising. The solid inoculum method was very successful but would be difficult to commercialize. Laboratory tests showed that higher temperatures around 30 °C could be used to speed up growth, but still longer incubation times were needed for pine. The current study was undertaken to determine the effect and feasibility of three different inoculation methods on the bioincising process: inoculation with grain colonized by D. squalens, a liquid spray inoculation (via dipping and via spray), and a mycelial mat (mycelium grown on the surface of agar and grown on paper). In addition the higher incubation temperature on 2x4 samples of both pine and spruce was tested at four, five and six weeks incubation for spruce, and six, seven and eight weeks incubation for pine. Results from the experiment suggest that liquid inoculum (spray) performs as well as grain inoculum. Spruce samples exposed for six weeks reached an average penetration of 10 mm through the heartwood face and 22 mm through the edge. Forty percent of the samples reached a minimum of 10 mm penetration on the heartwood face, and 80% reached 10 mm penetration through the edge. The ease of application via spraying (liquid inoculation method) makes this a more desirable method to use in an industrial setting. The greatest increases in preservative penetration were at six weeks in the grain inoculated samples; however, five and six week’s incubation for liquid inoculation were close. The results for bioincising on pine were much more variable, and increasing the incubation time did not substantially increase preservative penetration. This suggests that the fungal isolate used may not be suited to pine wood.