As methyl bromide is being phased out or severely restricted by 2015, and kiln-heating (56/30) is not ideal for some products, there is a need to find alternative treatments for phytosanitary purposes. Specific efficacy data against pests associated with wood products are needed if alternative treatments are to be recognized under ISPM-15 or ISPM-28. This study evaluates the efficacy of radio frequency (RF) energy heating for the treatment of pinewood nematode (PWN) infected wood. Following the most current guidelines on efficacy testing, in stage 1 of the study, 13 temperatures were tested on small wood samples to determine the minimum lethal temperature that produced 100% mortality of nematodes. The results showed 100% mortality at a minimum temperature of 56°C (based on Infra-red thermal images data 55.5-57.4). Additional samples were tested at 54°C, 56°C and 58°C to show 100% mortality at a sample size sufficient to satisfy the Probit 9 requirement where at least 93,613 individuals must be tested without survivors. In stage 2 of the experiment, RF heating was tested on industrial sized wood blocks. Due to observed variability in heating profile and the presence of cold spots in stage 1, in stage 2 two temperatures (58 and 60°C) were tested to ensure the samples reached the minimum lethal temperature. Survival was observed in only one sample, which, according to temperature probe data, did not maintain the minimum lethal temperature after a one minute hold time. Probit 9 was also satisfied in stage 2. The results of this study showed that to effectively treat PWN infected wood the entire sample must reach at least 56°C for a minimum of one minute using RF heating. Further work in the industry is needed to design schedules and technology able to deliver the target temperature thought the profile of treated wood in industrial scale operations.