Our work to address phytosanitary trade issues has focused on finding alternative methods of treating wood and wood products to eradicate pests of concern. Alternatives to methyl bromide require specific efficacy data against selection of pests associated with wood products, if these treatments are to be recognized under ISPM-15 or ISPM-28. In this study, dielectric heating with radio frequency (RF) energy was tested on lodgepole pine wood samples infected with various species of fungi, including bluestain, decay and mold species. We targeted the following temperatures: 40° 56°, 60°, 65° and 70°C with a hold time of one minute using the same test methodology as in our parallel study on RF efficacy against pine wood nematodes. Results for fungi were variable with no temperature resulting in 100% mortality of all fungal species. A Neurospora species, Fusarium solani, Oligoporus placenta and Antrodia carbonica showed some indication of heat tolerance as some replicates survived treatment at 60°C, 65°C and 70°C. Variable results among many of the isolates tested suggest that longer hold times or higher target temperatures should be tested in order to find effective temperature and time combinations for killing fungi in lodgepole pine using RF.