Literature searches were made with respect to the metabolites produced by species under consideration as biological control agents and other species of the same genera. Literature analytical methods were selected and developed to profile the organic solvent extracts of the selected fungi grown in standard media and to screen for specific metabolites by thin layer chromatography - mass spectrometry. From these screenings attempts were made to characterize specific components. Important classes of metabolites that might be expected to be produced by the candidateorganisms were identified from consideration of species believed to be related genetically through morphology and isozyme analysis. These include trichothecene-, cyclopentenylisocyanide-, and gliotoxin-type secondary metabolites and the peptaibol polypeptides. Screening of the candidate species demonstrated that these produce many metabolites, the profiles of which depend on the substrate on which they are grown. Few metabolites are readily identifiable on the basis of mass spectral data or retention indices alone. The Trichoderma strains were shown to produce dehydroacetic acid, a known antifungal agent, in defined media. T. viride EL3 was also shown to produce this metabolite on jack pine sapwood. All the Gliocladium strains of interest were shown to produce the Aib marker indicating the production of peptaibol antibiotics (mycotoxins). This is the first report of this type of metabolite for Gliocladium solani. For Gliocladium viride EL8 the production of 3,6-dibenzylpiperazine-2,5-dione was demonstrated. This indicates that this strain may have the potential to biosynthesize gliotoxin or related metabolites depending on culture conditions. The similarity between some of the metabolites reported to be produced by the genera under consideration as biocontrol agents and those reported to be produced by staining fungi may have implications with respect to the mechanisms of biocontrol.