In developing biocontrol technology against sapstain, Forintek identified ten potential biocontrol fungi, Identification of the metabolites that these leads produce will aid in understandng their mechanisms of biocontrol, facilitate product development, and is essential for regulatory acceptance. Identified as a concern with some of the lead fungi is the possiblity that they may produce mycotoxins of the trichothecene- or gliotoxin-type. ELISA's were developed for the detection of T-2 toxin. T-2 toxin was detected from a known producer, but it was not detected from the lead organisms under the growth conditions tested. Candidates were screened for production of gliotoxin and related metabolites by HPLC and TLC. EL 9 and 10 produced gliotoxin. EL 8 did not produce this mycotoxin, but a structurally-related metabolite. Siderophores have been implicated in resource capture and competition mechanisms of biocontrol. Universal and specific assays for the detection of siderophores was established. Production of hydroxamate-type siderophores was detected for all the leads, EL1 to EL10, when grown in liquid media. Production of siderophores by three staining fungi was also detected. Methods of isolation of siderophores in the iron-free and iron-complex forms and profiling by HPLC methods have been established. The characterization of these materials is continuing